The party of 33 included 29 individuals who were active participants in the Corps' organizational development, recruitment and training at its 1803–1804 winter staging area at Camp Dubois, Illinois Territory. They then departed from Camp Dubois, near present day Hartford, Illinois, and began their historic journey on May 14, 1804. They soon met up with Lewis in Saint Charles, Missouri, and the corps followed the Missouri River westward.
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